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KMID : 0380019950100030283
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Korean Journal of Biotechnology and Bioengineering
1995 Volume.10 No. 3 p.283 ~ p.290
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Continuous Production of Authentic Human Growth Hormone from Methionyl Human Growth Hormone Using the Column Reactor of Immobilized Aminopeptidase M
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이성희/Lee, Sung Hee
김기태/박범수/김은영/최차용/김원배/양중익/Kim, Ki Tae/Park, Beom Soo/Kim, Eun Young/Choi, Cha Yong/Kim, Won Bae/Yang, Jung Ick
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Abstract
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The characteristics of aminopeptidase M(ApM) immobilized covalently on Cellufine Formyl and the continuous production of authentic human growth hormone(hGH) from methionyl human growth hormone(met-hGH) using the column reactor packed with immobilized ApM were investigated. Immobilized ApM with the proportion of 2.3mg ApM per lg Cellufine Formyl gel had the highest met-hGH conversion activity. The optimum pH(7.0) and temperature(55t) showed no appreciable difference between free and immobilized enzymes and the optimum temperature in continuous operation of the column reactor was also found to be 55C. Under the conditions at which met-hGH was converted completely to hGH, the yield and productivity were about 77% and 0.8mg hGH/ml ? h, respectively. In two column reactors of different sizes, met-hGH was converted to hGH with the same conversion rates and hGH yields at the same space velocities. The half-life of the reactor systems at 45 *C and 551C were projected from the continuous operations for 90 days to be 225 days and 81 days, respectively.
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